ABSTRACT
Background: Today, the relationship between asthma and obesity in many studies is reviewed and weight control is used as a way for control of symptoms and severity of asthma along with other treatment protocol. Portulaca oleracea is consumed for weight loss in some countries
Objective: The experiment is designed to study the effect of Portulaca oleracea seeds on anthropometric measurements in asthmatic patients
Methods: In a randomized clinical trial, 59 patients with asthma disease divided to intervention [n=31] and control [n=28] groups. First group received asthma treatment and consisting 10g/day P. oleracea seeds in two doses in the morning [fasting] and before dinner with a glass of water for 8 weeks. Participants in second group received only asthma treatment. At the beginning and end of the study, anthropometric measurements were measured on each subject
Results: The anthropometric measurements including weight, body mass index and waist circumference indicated any significant differences between two groups after completion of the study [P?0.05] but hip circumference and waist-to-hip ratio had significant decrease [P= 0.003] and increase in intervention group [P= 0.04], respectively
Conclusion: Consumption of Portulaca oleracea seeds influence on some anthropometric measurements likes hip circumference and waist-to-hip ratio in asthmatic patients. However, the effects of them on other anthropometric measurements need further investigations.Keywords: Anthropometric Measurements, Asthma, Obesity, Portulaca oleracea seed
ABSTRACT
Malta fever is a zoonosis that it's causative agent is Brucella species. Although the disease is rarely fatal, but in natural epidemics or probable biologic wars, it's rapid and proper indiscrimination will scathe medical supplies drastically. Drawbacks of traditional diagnostic methods are time consuming, less accurate, less sensitive and contain false positive results. So the aim of this study is to design the PCR method for rapid detection of the organism. Genus specific bcsp target gene was selected for primer designing using Primer express v3.0 software. Specificity of the PCR tests was determined using reactions containing various bacterial negative control genomes. For evaluation of sensitivity and limit of detection, the PCR products were cloned in pTZ57R/T plasmid and used from their serial dilutions. Electrophoresis of bcsp gene PCR product showed a single 150 bp band for B.abortus and B.melitensis. PCR tests using negative control bacterial genomes were not showed detectable bands after gel agarose electrophoresis. The cloning processes were confirmed by PCR and sequencing. This method can be used for genus detection of the pathogen Brucella. and, it reduces time and cost needed for the organisms detection